ISOLASI, KARAKTERISASI, DAN PATOGENITAS BAKTERI PENYEBAB PENYAKIT PADA GURAMI (Osphronemus goramy) DI KABUPATEN BANTUL
Abstract: Gouramy
(Osphronemusgoramy) is an important fish comodity and preferred to be consumed
by people. One of the limiting factor in freshwater aquaculture is bacterial
infections. The objective of this research was to identify the species of
pathogenic bacteria on gourami from Bantul Regency and to determine their
pathogenicity. Eighteen diseased gouramy were collected from seven different fi
sh farms. Bacteria were isolated from kidney and purifyon TSA medium.
Characterization was conducted on the morphology of bacterial colonies and
cells, biochemical tests. Koch Postulate Test was done to confirm pathogenic
properties of the isolated bacteria. Pathogenicity test was performed by
intraperitoneal injection of a serial doses of bacteria into gouramy and the
Lethal Dosage 50 (LD50) was calculated. Twenty isolates of Aeromonas
hydrophila, 5 isolates of Aeromonas salmonicida and one isolate of Vibrio
cholerae,Vibrio anguillarum and Pseudomonas fluorescens respectively were
isolated from and confi rmed as pathogen ingouramy. Pathogenic diversity was
found among isolated bacteria, which a group of virulent bacteria as Aeromonas
hydrophila GBNB, GGRB, and GCMA3 with LD50 values of 6.28 x 105, 2.09 x 105 and
5.37 x 105 CFU /fish; and a group of non virulent bacteria as A. hydrophila GKRA
isolate and Vibrio cholera GPDA isolat with LD50 value of 7.14 x 107 and 3.45 x
107 CFU/fish respectively.
Keywords: characterization,
gouramy (Osphronemus goramy), identication, Koch’s postulate, pathogenic
bacteria
Penulis: Murwantoko, Rozi Rozi, Indah Istiqomah, Kamiso
H. Nitimulyo
Kode Jurnal: jpperikanandd130674
