INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION
Abstract: Very low naturally
mating rate of pond-reared tiger shrimp broodstock is probably due to the slow
maturation of the male stock. The aim of this study was to evaluate the salmon
gonadotrophin releasing hormone analoque (sGnRHa) in stimulating the gonadal
maturation of male stock of pond-reared tiger shrimp. The treatments were three
dosages of sGnRHa at 0.1 (OV-1), 0.2 (OV-2), and 0.3 (OV-3) mL/kg of shrimp
weight and control was eye stalk ablation (AB). The sGnRHa was administered via
injection three times with one week interval. Male stocks with average initial
body weight of 82.1 g were randomly distributed into four of 10 m3 concrete
tanks, 26 males for each tank. Variables observed were performances of
spermatophores and profiles of amino acid and fatty acid of muscle of the male
stocks. After induction, number of male maturing indicated by spermatophores
releasing from terminal ampullas was higher in shrimp induced with OV-1 (80.8%)
compared to control which was only 46.1%. Furthermore, shrimp treated OV-2 had
the highest spermatophore weight of 0.16 g compared to control (0.11 g) and
other two groups. Amino acid profiles improved as the dose of sGnRHa increased
up to 0.2 mL/kg from 61.23% for ablated male becoming 71.27% for OV-2. Total
fatty acid also tended to improve by increasing the dose of hormone injection,
however, the ablated male had higher total fatty acid content than that of
OV-1. The present finding demonstrated that the dose of sGnRHa to stimulate the
gonadal maturation of pond-reared male tiger shrimp could be applied at range
between 0.1-0.2 mL/kg of shrimp weight.
Keywords: sGnRHa; hormone
dosage; spermatophore; reproduction; tiger shrimp
Author: Asda Laining, Usman
Usman, Rachman Syah
Journal Code: jpperikanangg160051
