EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE
Abstract: The role of tiger
shrimp defense against invading pathogen on molecular level such antiviral gene
expression is limited to be reported. Gene expression is a process which codes
information of genes that is converted to the protein as a phenotype. Distribution
of PmAV antivirus gene, that has been reported as an important gene on
non-specific response immune, is needed to be observed to several
organs/tissues and size of tiger shrimp. The aim of this study is to determine
the distribution of gene antiviral expression at several organ/tissue and size
of shrimp. The organs/tissues observed in this study were: gill, hepatopancres,
muscle tissue, eyes, heart, stomach, gonad, and intestine. While the size of
shrimp consisted of three groups, those are: (A) 10-20 g/ind., (B) 30-40
g/ind., and (C) 60-70 g/ind. Analysis of antiviral gene expression was
performed by RNA extraction, followed by the cDNA syntesis, and amplification
of gene expression by semi-quantitative PCR. The result of PCR optimation
showed the optimal concentration of cDNA and primer was 1 μL and 50 mol, respectively for PCR final volume of 25 μL. Antiviral gene was
expressed on the hepatopancreas and stomach in percentage of 50.0% and 16.7%,
respectively. While the highest percentage of individual expressing the
antiviral gene was observed in the shrimp size of C (66.7%), followed by B
(50.0%) and A (16.7%). The result of study implied that the hepatopancreas has
importantly involed in tiger shrimp defense mechanism on viral infection.
Keywords: tiger shrimp; gene
expression; antiviral; organ tissue; size
Author: Andi Parenrengi, Andi
Tenriulo, Samuel Lante
Journal Code: jpperikanangg120034
