Chitosan Composite of Crab Shell and Hydroxyapatite of Tuna Fish Bone as Biomaterials for Guided Tissue Regeneration
Abstract: The needs of
membrane biomaterials to prevent the migration process of epithelial cells into
the bony area after tooth withdrawal process, for filler the holed tooth and to
cure gingivitis have increased significantly. In this study, a biomembranes
composed of chitosan (CTS) and hydroxyapatite (HA) were prepared. CTS was made
of crab shell and HA was made of tuna fish bone. There were 4 groups of
treatments, i.e.: K0: only 4 g CTS as a control; K1: combination of 4 g CTS and
2 g HA; K2: combination of 4 g CTS and 4 g HA; and K3: combination of 4 g CTS
and 6 g HA. The dried and solid membrane was soaked in 5% Na2SO3 for 1 hour,
and then dried in oven at 60 0C for 24 hours. The composite membrane
properties, i.e.: water absorption, biodegradation, tensile strength,
elongation at break, functional group (by FTIR), and microstructure (by SEM)
were evaluated. Result showed that the increasing of HA content in membrane
will decreasing the water absorption. The increasing of HA content in membrane
and soaking time in Phosphate Buffered Saline (PBS), can increase in biodegradation
rate. The biodegradation rate of membrane was about 4.44-6.01% during 27 days
of soaking time. The increasing soaking time in PBS can decrease the tensile
strength of membrane at about 18.33%-21.17% and decreased the elongation at
break at about 26.64%-59.94%. FT-IR analysis indicated that there was a
crosslinking between a CTS-HA composite membrane and Na2SO3. Among all the
composites studied, CTS-HA (4/2 g/g) composite membrane is the best one for
Guided Tissue Regeneration (GTR).
Keywords: biomembrane,
chitosan, hydroxyapatite; tensile strength; elongation at break
Author: Thamrin Wikanta,
Erizal Erizal, Sugiyono
Journal Code: jpperikanangg130018