Abstract: Deep sea is an extreme environment characterized by cold temperature, high pressure, lackof  light and nutrients. Microorganisms live in these habitat are unique microorganisms andknown to have tremendous source of potential agents for biotechnology processes. Indonesia asan archipelagic country has a vast deep ocean. This study aims to see the diversity of bacteria inSangihe Talaud Deep Sea, Sulawesi. Analysis of bacterial diversity was carried out by culturedand uncultured method. Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniquewas used for uncultured analysis of the microorganisms biodiversity, while cultured one wasdone by plating the samples of water onto Zobell media. The results showed that, there were 21isolates obtained by cultured method. The identification which based on 16S rDNA by PCR methodshowed the genus of Pseudomonas, Pseudoalteromonas, Alteromonas, Vibrio, Shewanella andUncultured bacterium were identified. However, 14 classes of bacteria were obtained by usingTRFLP method i.e Acetobacteraceae class, Actinobacteria, α-proteobacteria, -proteobacteria, δ-proteobacteria, γ-proteobacteria, Bacili, Bacteroidetes, Chlorobi, Chroococcales, Clostridia,Erysipelotrichi, Synergistia, and Zetaproteobacteria. here were also  unclassified bacteria anduncultured bacterium found in the samples.
Keywords: deep sea, diversity, bacteria, PCR, T-RFLP, Sangihe Talaud
Author: Gintung Patantis, Ekowati Chasanah, Dewi Seswita Zilda, Ikhsan B. Waluyo
Journal Code: jpperikanangg120015

Artikel Terkait :