ANDROGENESIS DIHASILKAN DARI BERBAGAI LAMA IRADIASI ULTRAVIOLET DAN BERBAGAI WAKTU KEJUT PANAS PASCA FERTILISASI PADA TELUR IKAN NILEM (Osteochilus hasselti CV)
Abstract: Androgenesis,
biotechnology involving the production of the whole individual chromosomes that
derived from the male brood stock as efforts to meet the quality of brood stock
fulfillment. Androgenesis involves two phases, the inactivation of the genetic
material of eggs, can be irradiated using ultraviolet (uv) rays and
diploidization of zygote stage using heat shock at 40oC. The study reported
androgenesis of shark minnow fish through two stages of various doses of UV
inactivation, followed by the diploidization at various lengths of times of
heat shock at 40oC after fertilization. Objectives of the research were to
determine: (1) the effectiveness of inactivation of TUV 254 nm phillips 15 watt
with 15 cm distance using various time of irradiation (as the dose) i.e: 1, 3,
or 5 minutes at shark minnow fish and 2) the effectiveness of diploidization by
heat shock at 40oC for 90 seconds at the various of time i.e: 15. 20 or 25
minutes after fertilization. This experimental study was complete randomized
block design, consisting of 13 treatment, i.e: 9 treatment androgenesis using
UV irradiation (1, 3 or 5 minute) and then followed by heat shock at 15, 20 or
25 minutes after fertilization (A1 - A9), and 4 treatment as a control that
consisting of 1 positive control, ie, without irradiation and without heat
shock (K0) and 3 negative controls were irradiated but not heat shock (K1, K2,
K3). Variables that be observed are the eggs fertilization, the degree of
hatching, the percentage of haploid larvae, the percentage of juvenile survival
rate after 28 days of culture. Data were analyzed with one way of variance
(ANOVA) using SPSS version 16.0 of Windows Software. The results showed that
the UV irradiation dose was effectively activating the genetic material of
shark minnow fish, where the most effective treatment was at 5 minutes (9916
J/m2). It proved that heat shock at 40°C for 90 seconds was effective to
prevent the first embryonic mitosis diploid androgenesis of shark minnow fish and
the most effective treatment was A9 with irradiation for 5 minute and heat
shock at 40°C for 90 seconds at 25 minutes after fertilization.
Keywords: androgenesis,
haploid larvae, heat shock, UV irradiation, shark minnow fi sh eggs
Penulis: Tiwuk Windari
Kode Jurnal: jpperikanandd130670