PENELUSURAN SENYAWA BIOAKTIF BAKTERI YANG BERASOSIASI DENGAN ANEMON LAUT SERTA IDENTIFIKASI MOLEKULERNYA BERBASIS 16S rDNA
Abstract: Sea
anemone-associated microorganisms are among of the most interesting and
promising marine natural product sources, which produce various biological
activities. In this study, marine bacteria were isolated from 2 kinds of sea
anemones, Entacmaea medusivora and Green Anemone that were collected from Bandengan,
Jepara and Maratua, East Borneo respectively. Nine bacterial isolatesof
E.medusivora were screened for antibacterial activity against 8 strains. One
out of 9 bacterial isolates was successfully screened and was found to be
active against V. parahaemolyticus strains. This active isolate was also
capable of amplifying PKS gene fragments necessary for the biosynthesis of
polyketide synthase. The identifi cation results revealed that the active
isolate is Bacillus cereus. One bacterial isolate of Green Anemone was screened
to produce the pigment and positively synthesize carotenoids. Initial analysis
with atomic absorption spectrophotometric method revealed that the wave length
of bacterial pigment were in the range of 300-600 nm, which are categorized that
within the group of carotenoid pigments. From the results of molecular identifi
cation by 16S rDNA method, it was shown that bacterium was closely related to
Xanthomonas sp.
Keywords: bacteria;
carotenoid; PKS-NRPS genes; sea anemone
Penulis: Naely K Wusqy, Dwi I
Prayitmo, Ocky K Radjasa, Leenawaty Limantara, Ferry F Karwur
Kode Jurnal: jpperikanandd120710