MUTAGENIC EFFECTS OF SODIUM AZIDE ON THE GERMINATION IN RICE (Oryza sativa L. cv. INPAGO UNSOED 1)
Abstract: The available
natural diversity highly limits the effort to improve the production of
upland-rice with delicious and aromatic flavors. However, the mutagenesis can
help improving the natural diversity. The aromatic-upland rice seeds, cv.
Inpago Unsoed 1, were soaked in solution of NaN3 with the doses of 0 mM, 1 mM,
2 mM, 3 mM, 4 mM, 5 mM, 6 mM, 7 mM, 8 mM, 9 mM, and 10 mM, in four replications
in completely randomized design. The observation included LD50, germination
percentage at first-count and final-count. Data were tabulated and analyzed
with CurveExpert 1.4 software for LD50, and generalized linear model in the
PROC-GLM procedure of SAS 9.1 software. The means were generated and compared
through Dunnett option, at probability level of 5%, in order to determine the
difference in means between mutated and non-mutated seeds. The results show
that the attributes of rice physiology affected by mutagenesis are sensitivity
of rice to NaN3 with LD50 at 8.84 mM, and significant effect of NaN3 on the
decreasing capacity of seed germination at > 6 mM for first count, and >
7 mM for final count.
Penulis: Bagus Herwibawa,
Florentina Kusmiyati
Kode Jurnal: jppertaniandd170570