GROWTH AND MORPHOLOGICAL CHANGES AS AN EARLY INDICATION OF IN VITRO PLOIDIZATION OF Tectona grandis
ABSTRACT: Ploidization level
estimation can be conducted in several methods, through morphological, growth
response, anatomy, cytology, and
molecular markers. The simplest and easiest methods are morphological marker
and growth response. The study aimed to develop early detection method of
polyploidy occurrence in in vitro. Tectona grandis after treated by antimitotic
agent colchicine. Nodal segments were immersed at 0, 15, and 30 μM colchicines
for 5 days, then cultured for 8 weeks. Observations on plantlet height, number
of leaves and morphology were performed at 2, 4, and 6 weeks after planting.
Colchicine had high significant effect on the height and significant effect on
leaves number. High concentration colchicine inhibited shoot elongation and
leaves growth, however it increased morphological changes. The planlets height
of 0, 15, and 30 μM of colchicine treatment was 4.14; 3.82; 3.12 cm; while the
number of leaves as much as 8.72; 8.4, and 7.5. Colchicine led to increase in
morphological changes at the levels 0, 15, 30 μM were 26,60%; 46.66%; and
93.33%. Changes caused by polyploidy differ from media. Changes in polyploidy
decreased the height, number of leaves, and induced morphological changes,
whereas planting media resulted in vitrification. Response to colchicines in
culture of T. grandis plantlet allows the growth and morphology to be a marker
for early detection of polyploidization.
Kata Kunci: Tectona grandis;
colchicine; polyploidy detection
Penulis: Yusuf Sigit Ahmad
Fauzan, Supriyanto, Teuku Tajuddin
Kode Jurnal: jpkehutanandd170274
