X-ray radiation effect of C-arm on adipose tissue-mesenchymal stem cell viability and population doubling time
Abstract: Adipose tissue
derived mesenchymal stem cells (AT-MSCs) are relatively easy in isolation
procedure compared to bone marrow-derived. Minimally invasive MSC injections
need C-arm as guidance that potentially influence the cell viability and doubling
time. This study aimsed to determine the effect of C-arm X-ray exposure on
AT-MSC viability and population doubling time (PDT).
Methods: This experimental study used cryopreserved adipose tissue
derived MSCs stored in Stem Cell Medical Technology Integrated Service Unit
Cipto Mangunkusumo Hospital. Cells were thawed, propagated, and exposed to
varying doses of C-arm X-ray radiation. Stem cell viability was measured, and
then the cells were cultured to assess their PDT. Generalized linear models
test was used to compare cell viability between post-thaw, post-propagation,
post-radiation, post-culture post-radiation, and control and between radiation
dose groups. Kruskal-Wallis test assessed PDT between various radiation doses
in post-radiation groups. Wilcoxon test was used to assess PDT between
pre-radiation and post-radiation groups.
Results: Mean confluence period of adipose MSCs post- irradiation was
4.33 days. There was no statistically significant difference in MSC viability
after X-ray exposure between pre- and post-irradiation groups (p=0.831). There
was no correlation between post-irradiation viability and radiation dose
(p=0.138, r=0.503). There were no significant differences in PDT between pre-
and post-culture post-irradiation groups and between various radiation doses in
post-irradiation groups (p=0.792).
Conclusion: MSC viability and PDT were not influenced by radiation
exposure up to 32.34 mgray.
Author: Ahmad J. Rahyussalim,
Jeanne A. Pawitan, Alam R. Kusnadi, Tri Kurniawati
Journal Code: jpkedokterangg160147
