Vitamin D Prevents Endothelial Damage Induced by Increased Neutrophil Extracellular Traps Formation in Patients with Systemic Lupus Erythematosus
ABSTRACT: to investigate the
effects of Vitamin D calcitriol/1,25(OH)2D3 on NETosis in systemic lupus erythematosus
(SLE) patients with hypovitamin D. Methods: neutrophlis of fve SLE patients
with hypovitamin D were divided into 4 groups, P0 (0 nM/control), P1 (1 nM), P2
(10 nM), and P3 (100 nM) as cultured samples. Phorbol Myristate Acetate (PMA)
was used to stimulate NETs formation. The supernatant was separated and cocultured
with HUVECs. Externalization of Neutrophil Elastase (NE) and Myeloperoxidase
(MPO) during NETosis was measured by immunofluorescence and ELISA respectively.
Early and late apoptosis of endothelial cell was measured by flowcytometry using
cell death kit (Annexin V and PI antibody). Results: this study showed signifcant
decrease in early apoptosis with 10 nM of 1,25(OH)2D3 compared to control
group. Signifcance of NE externalization found in all treatment groups
(p<0.05), while MPO absorbance in the same tendency but not statistically
signifcant. Further analysis also found a moderate positive correlation between
NE externalizations with early apoptosis. Conclusion: vitamin D 1,25(OH)2D3
could reduce endothelial damage by decreasing NETosis activity. This result may
reveal the possibility of Vitamin D as supplementary therapy for SLE patients with
hypovitamin D to prevent endothelial damage.
Author: Kusworini Handono,
Yona One Sidarta, Benny A. Pradana, Radhitio A. Nugroho, Ivan A. Hartono,
Handono Kalim, Agustina T. Endharti
Journal Code: jpkedokterangg140281