Use of Pleural Fluid Interferon-gamma Enzyme-linked Immunospot Assay in the Diagnosis of Pleural Tuberculosis
ABSTRACT: to evaluate the
diagnostic value of an interferon-gamma release assay (IGRA) with enzyme-linked
immunospot (ELISPOT) method, T-SPOT.TB, in the diagnosis of pleural TB using
pleural fluid mononuclear cells (PFMC). Methods: forty-eight subjects, presumed
to have pleural TB with exudative pleural effusion by Light’s criteria,
dominated by mononuclear cells, had their pleural fluid specimen tested with
T-SPOT. TB, Mycobacterial Growth Indicator Tube (MGIT) culture, and adenosine
deaminase (ADA) activity. Other causes of pleural effusion such as heart
failure, renal failure, hepatic cirrhosis, and malignancy were excluded. Results:
the sensitivity, specifcity, positive predictive value, and negative predictive
value of the IGRA ELISPOT assay using PFMC for the diagnosis of pleural TB were
100%, 88.89%, 97.5%, and 100%, respectively. Conclusion: IGRA with ELISPOT
method performed on PFMC is useful for a rapid and reliable diagnosis of pleural
TB in clinical practice, especially in area with high TB burden.
Author: Tika Adilistya, Dalima
A.W. Astrawinata, Ujainah Z. Nasir
Journal Code: jpkedokterangg160013
