The percentage of macrophage numbers in rat model of sciatic nerve crush injury
Abstract: Excessive
accumulation of macrophages in sciatic nerve fascicles inhibits regeneration of
peripheral nerves. The aim of this study is to determine the percentage of the
macrophages inside and outside of the fascicles at the proximal, at the site of
injury and at the distal segment of rat model of sciatic nerve crush injury.
Thirty male 3 months age Wistar rats of 200-230 g were divided into
sham-operation group and crush injury group. Termination was performed on day
3, 7, and 14 after crush injury. Immunohistochemical examination was done using
anti CD68 antibody. Counting of immunopositive and immunonegative cells was
done on three representative fields for extrafascicular and intrafascicular
area of proximal, injury and distal segments. The data was presented as
percentage of immunopositive cells. The percentage of the macrophages was
significantly increased in crush injury group compared to the sham-operated
group in all segments of the peripheral nerves. While the percentage of
macrophages outside fascicle in all segments of sciatic nerve and within the
fascicle in the proximal segment reached its peak on day 3, the percentage of
macrophages within the fascicles at the site of injury and distal segments
reached the peak later at day 7. In conclusions, accumulation of macrophages
outside the nerve fascicles occurs at the beginning of the injury, and then
followed later by the accumulation of macrophages within nerve fascicles.
Author: Satrio Wicaksono,
Muhammad Ghufron, Rina Susilowati
Journal Code: jpkedokterangg150351
