The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7
ABSTRACT: The cytotoxic
activity of a-terpineol on T47D and HeLa cancer cell lines have been reported.
This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis
and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity
of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were
analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using
immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on
the MCF-7 cell lines with an IC 50 value of 33.0 ± 5.4 μg/mL. a-Terpineol
induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell.
Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion,
a-terpineol has potential anticancer activity against MCF-7 cancer cell line
trough through cells cycle inhibition and apoptosis stimulation.
Author: Damiana Sapta
Candrasari, Sofia Mubarika, Mae Sri Hartati Wahyuningsih
Journal Code: jpkedokterangg150350
