THE EFFECT OF PROPOLIS EXTRACT ON MDA LEVELS (MALONDIALDEHYDE) AND SPERM QUALITY ON EPIDIDIMIS
ABSTRACT: Cigarette smoke
exposure increase free radicals and effecting male infertility. Propolis is
commonly used as supplement to increase antioxidant levels.
Objectives: To prove the effect of propolis extract to MDA level and
spermatozoa quality on epididymis due to cigarette smokes.
Methods: Experimental research post test only control group design using
30 male rats Wistar Strain, 8 weeks old, weighed 200 – 250 gram, randomly
divided into 5 groups. K-1 (normal), do not received propolis and cigarettes
smoke; K-2 (negative control) exposed to cigarettes smokes 4 buds/ day, did not
received propolis; K-3, K-4, and K-5, apart from cigarrete smokes 4 buds/day
each were given propolis with 2.9 mg, 5.4 mg, dan 8.3 mg/200g body weight/day
for 21 days. On the 22nd day, rats were sacrificed, sperms were extracted from
cauda epididymis, and then examined for MDA and sperm quality. Statistical
analysis used was one way ANOVA, continued with post hoc LSD with significance
level of p<0,05.
Results: ANOVA test on MDA level and sperm quality between groups showed
significant difference of, p = 0.005. Post hoc test indicated that MDA on K-5
(1,12) is significantly lower compare to K-2 (5,89), p = 0,001. As well as
consentration, motility and morphology (spermatozoa quality) which indicated
that K-5 (49.32, 64.15, 36.34) is significantly higher compared to K-2 (22.62,
27.27, 21.39), each with p value of p = 0.001.
Conclusion: extract propolis 8,3 mg/day for 21 days is proven to increase
spermatozoa quality and lower the MDA level on male rats wistar strains’
epididimis exposed to cigarettes smoke.
Author: I. Hoesada, T.
Nasihun, I. Isradji
Journal Code: jpkedokterangg160049