The Effect of PRF on Serum Starved Human Dermal Fibroblast.
Abstract: Healing failure on chronic ulcers was suspected due to the decrease of Growth Factors (GFs) supply, caused by either GFs trapped in the fibrin, or degraded by protease, or decreased level due to reduction of GFs gene expression. Administration of various GFs can stimulate healing of chronic ulcers. High level of GFs is available in biologic material called Platelet Rich Fibrin (PRF).
Objectives: To have in vitro evidence of PRF effect on GFs-serum starved human dermal fibroblasts as representative cells of chronic ulcers.
Methods: Human dermal fibroblasts (HDFs) were isolated from foreskin of six boys aged 11- 14 years-old. After 24 hours of serum deprivation, HDFs were treated by 100%, 50%, and 25% PRF lysate diluted in cultured medium. Cellular migration was measured using scratch assay, while cellular viability was using MTT assay and collagen deposition was using sirius red assay.
Results: The HDFs of serum starvation group showed significant impairment activities (p<0.05) in terms of cellular migration (25%), cellular proliferation (20%), and collagen deposition (10%). Administration with various levels of PRF lysate could significantly (p < 0.05) recover those activities. Because cellular activities of serum starved HDFs is similar with fibroblasts isolated from the bottom of chronic ulcers and administration of various levels of PRF lysate was capable to recover those activities, it can be concluded that PRF is a good biologic material to stimulate healing of chronic ulcers. However, in order to get better evidence based medicine, clinical trials must still be performed.
Author: Sunardi Radiono, Yohanes Widodo Wirohadidjojo, Arief Budiyanto
Journal Code: jpkedokterangg160184