The Effect of PRF on Serum Starved Human Dermal Fibroblast.
Abstract: Healing failure on
chronic ulcers was suspected due to the decrease of Growth Factors (GFs)
supply, caused by either GFs trapped in the fibrin, or degraded by protease, or
decreased level due to reduction of GFs gene expression. Administration of
various GFs can stimulate healing of chronic ulcers. High level of GFs is
available in biologic material called Platelet Rich Fibrin (PRF).
Objectives: To have in vitro evidence of PRF effect on GFs-serum starved
human dermal fibroblasts as representative cells of chronic ulcers.
Methods: Human dermal fibroblasts (HDFs) were isolated from foreskin of
six boys aged 11- 14 years-old. After 24 hours of serum deprivation, HDFs were
treated by 100%, 50%, and 25% PRF lysate diluted in cultured medium. Cellular
migration was measured using scratch assay, while cellular viability was using
MTT assay and collagen deposition was using sirius red assay.
Results: The HDFs of serum starvation group showed significant impairment
activities (p<0.05) in terms of cellular migration (25%), cellular
proliferation (20%), and collagen deposition (10%). Administration with various
levels of PRF lysate could significantly (p < 0.05) recover those
activities. Because cellular activities of serum starved HDFs is similar with
fibroblasts isolated from the bottom of chronic ulcers and administration of
various levels of PRF lysate was capable to recover those activities, it can be
concluded that PRF is a good biologic material to stimulate healing of chronic
ulcers. However, in order to get better evidence based medicine, clinical
trials must still be performed.
Author: Sunardi Radiono,
Yohanes Widodo Wirohadidjojo, Arief Budiyanto
Journal Code: jpkedokterangg160184