The effect of mitomycin-c in keloid fibroblast cultures
ABSTRACT: Keloid occurs due to
hyperactivity of Keloid Fibroblast (KF) in proliferation, migration, collagen
deposition, together with low rates of collagen degradation. These are under
the responsibility of TGF-β. Mitomycin C (MC) is used for treating keloid by a
topical application during surgery at the level of 0.02% to 0.08%.
Unfortunately, the lowest effective level of MC for keloid has not been
determined yet. We aimed to determine the lowest effective level of MC in the
suppression of KF activities. Various levels of MC diluted in growth medium
were administered on KF that were isolated from six patients. After 24 hours
and 72 hours of incubation, cellular proliferation, collagen deposition,
cellular migration and level of TGF-β, were analyzed. Application of 120 uM MC
on KF culture for 24 hours could significantly reduce TGF-β production from
1265.74 ± 274.81 pg/mL to 265.17 ± 12.20 pg/mL; proliferation index from 100%
to 84.01 ± 12.91%; inhibit cellular migration to 64.38 ± 3.66%; but reduce
collagen depositions from 100% to only 91.13 ± 10.19%. The lowest MC level is
on 30 uM or equal with 0.001%. In conclusion, the lowest level of MC can
suppress the activities of KF is 0.001%. Moreover, due to low activity in
inhibiting collagen deposition, MC would be better as an adjuvant drug for
keloid surgery.
Author: Ishandono Dachlan,
Teguh Aryandono, Mae Sri Hartati Wahyuningsih, Hardyanto Soebono, Yohanes
Widodo Wirohadidjojo
Journal Code: jpkedokterangg160187
