The cytotoxic effect of pinostrobin fingerroot (boesenbergia Apandurata)on the culture of hela cells
Abstract: Cancer is one of the
leading causes of death worldwide, therefore struggles to find more effective
treatment and prevention is needed. Several studies has been performed using
natural ingredients, one of which is Temu Kunci (B. pandurata). Temu Kunci
extract contains flavonoid pinostrobin that has been showed as having
cytotoxicity effects. Cytotoxicity tests of pinostrobin have been performed on several
tumor cell lines, but its cytotoxicity effect on HeLa cell line has never been
reported.
Objective: To assess cytotoxicity effect of pinostrobin temu kunci on HeLa
cell culture
Methods: This study used simple experimental design. Pinostrobin were
isolated from temu kunci and proved by TLC densitometry compared to standard
pinostrobin. HeLa cell culture were treated with pinostrobin with
concentrations 5, 25, 50, 75, 100, and 250 ug/mL. Cytotoxicity test were
performed by MTT assay. Data were analyzed using one-way ANOVA.
Results: There was significant difference (p=0.000) of means of cell viability
percentage, respectively: 92.58 ± 9.84 (5µg/mL), 91.78 ± 4.4 (25µg/mL), 80.09 ±
4.51 (50µg/mL), 76.89 ± 7.75 (75µg/mL), 67.85 ± 11.31 (100µg/mL), dan 48.82 ±
16.61 (250µg/mL). The IC50 was 250µg/mL.
Conclusion: Pinostrobin showed no active cytotoxicity effect on HeLa cell
culture.
Author: Rosmelia, Betty
Ekawati Suryaningsih, Hady Anshory, Yohanes Widodo Wirohadidjojo
Journal Code: jpkedokterangg160168
