Suplementasi Hormon Gonadotropin Pada Medium Maturasi In Vitro Untuk Meningkatkan Perkembangan Embrio Stadium 4 Sel Kambing Bligon
Abstract: The study was
carried out to investigate the effect of gonadotropin hormone supplementation
into in vitro maturation medium on maturation, fertilization and embryo
development of Bligon goats. This research steps consist of oocyte collection,
in vitro maturation, in vitro fertilization, and in vitro embryo development.
At the maturation stage the oocyte that had been collected and divided into two
groups based on the maturation medium, that was tissue culture medium (TCM)
with supplementation of GnRH 0 IU/mL and GnRH 25 IU/mL. Oocyte and embryo morphology
data were analyzed descriptively. Maturation rate and embryo development data
were analyzed by using independent sample t-test. Fertilization data was
analyzed descriptively. The result showed the percentages of mature oocytes
from gonadotropin supplementation of 0 IU/mL and 25 IU/mL were 54.10±25.97 and
54.89±26.44%, respectively. Expansion cumulus cells surrounding the oocytes
might indicated the mature oocytes. Cleavage rate of the 2 cells stage were
13,02±11,09 and 27,01±16,65%; respectively, and for the 4 cells stage were
10,16±10,01% and 16,67±14.91%. Embryos obtained from the treatment, indicated
uniform of blastomeres in the size, tight, compact, intact, and round-spherical
shape. It could be concluded that supplementation of gonadotropin hormone into
in vitro maturation medium could not increase the rate of oocyte maturation and
4 cell embryo development, but it could increase 2 cell embryo development of
Bligon goats. Hormone supplementation could improved the maturation and embryo
quality.
Penulis: Dewi Pranatasari,
Kustono, Diah Tri Widayati
Kode Jurnal: jppeternakandd160073
