Serum vitamin D and vitamin D receptor gene FokI polymorphisms in children with tuberculosis
Abstract: Background Vitamin D
deficiency and vitamin D receptor (VDR) gene polymorphisms are strongly
associated with tuberculosis (TB) susceptibility in countries with four
seasons. As a country with sufficient sunlight for vitamin D production in
skin, the incidence of TB in Indonesia remains high. Objective To assess for
possible associations between the incidence of tuberculosis and serum vitamin D
level, as well as VDR FokI polymorphisms in children. Methods A case-control
study was conducted at the Department of Child Health, Dr. Mohammad Hoesin
Hospital, Palembang from November 2011 to April 2012. Subjects were children
with TB (the case) and children without TB who had been exposed to TB in the
home (the control). Serum vitamin D [1,25(OH)2D3 or calcitriol] level was
measured by immunodiagnostic system (IDS) 1,25-dihydroxy vitamin D enzyme
immunoassay (EIA) kit. The VDR FokI polymorphisms were identified by polymerase
chain reaction (PCR) and restriction-fragment length polymorphism (RFLP)
analysis. Results Sixty subjects was divided equally into the case and control
groups. The mean serum calcitriol level in the case group was significantly
lower than that of the control group [105.5 (SD 66.9) pmol/L vs. 162.9 (SD 52.9)
pmol/L, respectively; (P=0.001)]. We found 9 subjects with calcitriol
deficiency, 8 in the TB group and 1 in the healthy contact group (OR 10.5;
95%CI 1.2 to 90.7) The VDR FokI polymorphism was seen in 28 subjects in the
case group and 22 in the control group (OR 5.0; 95%CI 0.9 to 26.4). Conclusion
Vitamin D (calcitriol) deficiency and lower serum levels are associated with
higher risk of TB in children. The VDR gene FokI polymorphism also contributes
to susceptibility for TB.
Author: Ariesta Karmila,
Muhammad Nazir, Kiagus Yangtjik, Yuwono
Journal Code: jpkedokterangg150265
