Response of Long-Term Memory to Molecular Changes of BDNF in Hippocampus in Various Intensities of Physical Activity
Abstract: To analyze the
physiological response of long-term memory (LTM) to the molecular changes of
brain-derived neurotrophic factor (BDNF) in the hippocampus by the treatment of
various intensities of physical activity.
Methods: Subjects were 7–8 week old male Wistar rats weighed between
201–250 grams. This study was an experimental study with pre-(day-1) and
post-(day-14) design. Molecular changes reflected by the changes in the
expression of mRNA and protein of BDNF in the hippocampus. Treatment of
physical activity on the subjects was running on the Animal Treadmill by
grouping of the physical activity: light intensity at a speed of 10 m/min,
moderate intensity at a speed of 20 m/min and heavy intensity at a speed of 30
m/min. The treatment’s duration was 30 minutes.Then, analysis of data on pre
(day-1) and post (day-14) which were: LTM response based on travel time
swimming test, the expression of mRNA (Ct) and protein (%) of BDNF in
hippocampus based on RT-PCR and immunohistochemistry.
Results: The results showed that moderate intensity group caused the better
physiological and molecular responses than the other groups, as follows: travel
time (0.6260 vs 0.7270 vs 0.9400 vs. 1.4000 seconds (p<0.05)), mRNA BDNF
expression (17.2320 vs 18.8800 vs 19.7540 vs 20.7750 Ct (p<0.05)), and
hippocampal BDNF protein expression.
Conclusions: The study conclude that the moderate intensity is the best
physical activity to improve LTM as showed by the BDNF mRNA expression as well
as BDNF protein in hippocampus.
Author: Leonardo Lubis,
Setiawan
Journal Code: jpkedokterangg160128
