Repellency Test of Wet Tissue Containing DEET (N,N-diethyl- 3-metatoluamide) and Citronella Oil (Cymbopogon citratus) Againts Aedes Aegypti Mosquitoes
Abstract: Dengue hemorrhagic
fever has been one of the major health problems in Indonesia. As the disease
spreads out by Aedes aegypti, a variety of ways has been conducted to
disconnect host, agent, and the environment chain including prevention of human
contact with the vector using by various repellents. The present test compared
the complete protection time of N,N-diethyl- 3-metatoluamide (DEET) and
citronella oil impregnated in wet tissue against Aedes aegypti. For this test,
an ethanol-based 12.5% DEET and 20% citronella oil were prepared, into which
dry tissue papers were immersed, drain in open air, and then stored in aluminum
foil packs for one day and one week. Meanwhile, a number of disease-free adult
female Aedes aegypti were placed in mosquito cages. The prepared one-day and
oneweek stored wet tissues were used to swab volunteer adult human hands (from
elbow to wrist) which were then inserted into the mosquito cage in three
replicates (25 mosquitoes each cage). Elapsed time from first hand insertion to
the first mosquito bite was calculated and expressed as repellency time of the
impregnated repellent. It was found that the average repellency times of
one-day stored 12.5% DEET and 20% citronella oil wet tissues were 4 hour 26
minutes and 14.24 minutes, respectively, while for oneweek stored were 4 hour 6
minutes and 12.57 minutes, respectively. Post Hoc test showed that the
repellency time difference between one-day and one-week storage was not
statistically significance (p = 0.524 for DEET and p = 0.681 for citronella
oil).
Penulis: Tri Baskoro T.
Satoto, Budi Mulyaningsih, M.M. Sintorini, A.F. Sugiarto, B.A Kesuma
Kode Jurnal: jpkesmasdd110211
