Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice
Abstract: Lead is able to pass
through the placental barrier and interfere with fetal development. Red fruit
has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols
(vitamin E) and unsaturated fatty acids. This study aims to examine the effect
of red fruit oil towards placenta in pregnant mice before lead exposure.
This was a laboratory experimental post test only study, using 20
pregnant mice (Mus musculus) that were randomized into five groups. The
negative control group was not exposed to lead; intervention group 0 (P0) was
exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The
intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day,
0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure.
Normal trophoblast cell count and caspase-9 expression of trophoblasts were
calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to
analyze the data.
The intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day
and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell
count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8%
compared with P0 to which was not given red fruit (p=0.020).
Red fruit increased normal trophoblast cell count by 88.2% and decreased
caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should
be noted that red fuit may prevent oxidative damage to cells.
Author: Portia Sumarsono, Widjiati,
Suherni Susilowati
Journal Code: jpkedokterangg160044
