Profle of PRMT-1 Gene Polymorphism in Hemodialysis Patients with Increased ADMA Levels
ABSTRACT: to determine the
distribution of PRMT-1 gene polymorphism and ADMA levels among continuing hemodialysis
patients. Methods: genotyping of PRMT-1 polymorphism was performed in 57
hemodialysis patients at Al Irsjad Hospital. All participants were recruited
for physical examination, questionnaire, and collection of 5 mL fasting venous
blood. The blood was treated with phenol-chloroform extraction of genomic DNA.
The candidate’s single nucleotide polymorphisms (SNPs) were genotyped by using
polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
The ADMA plasma levels was determined by ELISA and all biochemical indicators
of serum were examined. Results: ffty-seven hemodialysis patients participated
in our study, 54 (95.4%) of them had increased ADMA plasma levels. DNA
sequencing analysis of 13 patients showed a suspected PRMT-1 gene polymorphism
at sequence 5837 as there were different genotypes between C and G. Conclusion:
the increased levels of ADMA might be caused by PRMT-1 gene polymorphism.
Author: Mochammad Taha, Wenny
P. Nilamsari, Mochammad Yusuf, M. Amin
Journal Code: jpkedokterangg140274
