Prefrontal cortex cell proliferation of adult rats after chronic stress treated with ethanolic extract of Centella asiatica (L) Urban.
ABSTRACT: The decrease of
proliferation level of cells in several regions of adult brain is found after stress
exposure. One of such area is prefrontal cortex. Herbal medicine as antistress has
been used widely. Centella asiatica (L) Urban extract was reported to have
potent compounds to increase brain function. The objective of this study is to
investigate the effects of ethanolic extract C. asiatica on cell proliferation
of the adult prefrontal cortex in rats (PFC) after chronic stress. Adult male
Sprague-Dawley rats, weighing 260-390 g were randomly assigned into six
experimental groups, with fve rats per group, i.e. Group 1 as nomal control
without chronic stress, Group 2 as stress control, Group 3 aspositive control
given fluoxetine, Group 4-6 as treatment groups given 150; 300 and 600 of
ethanolic extrac C. asiatica, respectively. Extract were administered orally to
the rats following a period of restraint duration of 6 hours/day for 21 days.
Bromodeoxyuridine (BrdU) immunohistochemistry was used to label the
proliferated cells. Physical fractionator method was used to estimate the total
number of proliferated cells. One-way analysis of variance (ANOVA) followed by
Tukey post hoc test was used to evaluate the differenc between groups.
BrdU-labeled cells on medial prefrontal cortex were as follows: 1715.3±1345.1
(Group 1), 2659.2±2250.6 (Group 2), 4077.4±2415.3 (Group 3), 1784.1±908.3
(Group 4), 3056.6±4263.3 (Group 5), and 2153.4±2259.4 (Group 6). No signifcance
difference between groups was observed (p>0.05). In conclusion, the administration
of ethanolic extract of C. asiatica does not influence cell proliferation on prefrontal
cortex of rats after chronic stress.
Author: Yuniasih Mulyani
Jubeliene Taihuttu, Ginus Partadiredja, Dwi Cahyani Ratna Sari
Journal Code: jpkedokterangg160180
