Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011

ABSTRACT: Aim: the goal of this study is to understand the phenotype characteristic of beta-lactamase enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods: three different methods are performed to confrm those phenotypic characteristics, namely double disk diffusion method to confrm ESBL, AmpC disk test (cefoxitin-based) to confrm AmpC, and modifed Hodge test to confrm carbapenemase. Results: using double disk diffusion method, we found 58.42% isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98% are AmpC-producing. By conducting modifed Hodge test (MHT), 27.59% isolates are confrmed as carbapenemase-producing bacteria. Conclusion: this study confrmed the prevalence of betalactamase producing Klebsiella pneumoniae is very high. Nevertheless, AmpC and carbapenemase should not be ignored despite their low prevalence.
Key words: Enterobacteriaceae, antibiotic resistance, ESBL, AmpC, carbapenemase
Author: Yulia R. Saharman, Delly C. Lestari
Journal Code: jpkedokterangg130248