Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011
ABSTRACT: Aim: the goal of
this study is to understand the phenotype characteristic of beta-lactamase
enzymes producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase.
Methods: three different methods are performed to confrm those phenotypic
characteristics, namely double disk diffusion method to confrm ESBL, AmpC disk
test (cefoxitin-based) to confrm AmpC, and modifed Hodge test to confrm
carbapenemase. Results: using double disk diffusion method, we found 58.42%
isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98%
are AmpC-producing. By conducting modifed Hodge test (MHT), 27.59% isolates are
confrmed as carbapenemase-producing bacteria. Conclusion: this study confrmed
the prevalence of betalactamase producing Klebsiella pneumoniae is very high.
Nevertheless, AmpC and carbapenemase should not be ignored despite their low
prevalence.
Author: Yulia R. Saharman,
Delly C. Lestari
Journal Code: jpkedokterangg130248
