Peningkatan aktivitas fagositosis dan produksi nitrit oksida pada makrofag peritoneum tikus Sprague Dawley yang diberi Lactobacillus plantarum Mut7 dan ekstrak serat ubi jalar
Abstract: Macrophages play an
important role as part of the innate immune response in the gut and they
represent one of the first lines of nonspecific defense against bacterial
invasion. Previous studies indicated that probiotics and prebiotics may act as
an immunomodulator agents. Nevertheless, research on the immunomodulatory
effect of local materials has never been performed.
Objective: To study the effects of Lactobacillus plantarum Mut7 and sweet
potato fiber on the activity and Nitric Oxide (NO) production of peritoneal
macrophages of Sprague Dawley rats.
Method: Ninety six Sprague Dawley rats aged 8 weeks were divided into two
groups; A (not infected with Salmonella typhimurium) and B (infected with S.
typhimurium). Each group was divided into 4 subgroups and assigned to standard
AIN-93M diet (KON), 109 CFU/ml of L. plantarum Mut7 (PRO), modified AIN-93M
diet with sweet potato fiber (PRE), and both component (SIN). After 3 weeks of
treatment, 6 rats of each subgroup were sacrificed and the peritoneal
macrophages were isolated and analysed for its activity and NO production. The
rest of the rats continued the treatments for another 2 weeks. At the end of
the experiment, they were sacrificed and the peritoneal macrophage were
isolated and analysed for its activity and NO production.
Results: Oral administration of L.
plantarum Mut7, sweet potato fiber, or both improve phagocytic activity of
peritoneal macrophage which was indicated by an increase in the percentage of
macrophages that phagocyte latex particles (p<0.05) and an increase in the
number of latex particles engulfed by macrophages either after 3 or 5 weeks of
treatment (p<0.05). Oral administration of L. plantarum Mut7, sweet potato
fiber, or both were unable to increase the nitric oxide production after 3
weeks of treatment (p>0.05), but after 5 weeks of treatment the production
of NO was significantly increased (p<0.05).
Conclusion: L. plantarum Mut7, sweet potato fiber, or both increase the
non specific immune response as they could improve the activity and NO
production of peritoneal macrophages.
Penulis: Lily Arsanti Lestari,
Marsetyawan Heparis Nur Ekandaru Soesatyo, Susi Iravati, Eni Harmayani
Kode Jurnal: jpkesmasdd120428