Partial Sequencing of 16S rRNA Gene of Selected Staphylococcus aureus Isolates and its Antibiotic Resistance
Abstract: The choice of primer
used in 16S rRNA sequencing for identification of Staphylococcus species found
in food is important. This study aimed to characterize Staphylococcus aureus
isolates by partial sequencing based on 16S rRNA gene employing primers 16sF,
63F or 1387R. The isolates were isolated from milk, egg dishes and chicken
dishes and selected based on the presence of sea gene that responsible for
formation of enterotoxin-A. Antibiotic susceptibility of the isolates towards
six antibiotics was also tested. The use of 16sF resulted generally in higher
identity percentage and query coverage compared to the sequencing by 63F or
1387R. BLAST results of all isolates, sequenced by 16sF, showed 99% homology to
complete genome of four S. aureus strains, with different characteristics on enterotoxin
production and antibiotic resistance. Considering that all isolates were
carrying sea gene, indicated by the occurence of 120 bp amplicon after PCR
amplification using primer SEA1/SEA2,
the isolates were most in agreeing to S. aureus subsp. aureus ST288.
This study indicated that 4 out of 8 selected isolates were resistant towards
streptomycin. The 16S rRNA gene sequencing using 16sF is useful for
identification of S. aureus. However, additional analysis such as PCR employing
specific gene target, should give a valuable supplementary information, when
specific characteristic is expected.
Author: Harsi Dewantari
Kusumaningrum, Lita Handayani, Reni Nofrianti
Journal Code: jppeternakangg160044
