Abstract: The SNP (rs 42688595) in LOC514211 is uncharacterized gene and located in chromosome 13 in Bos taurus, had significant effects on milk yield and milk quality that have been identified from Chinese and British dairy cows using GWAS (Genome-wide Association Study) based on 60k SNP-Chip in the previous study. The objective of this study was to confirm the effects of the SNP in LOC514211 on productive traits in another population of Chinese Holstein dairy cow. Four hundred and seventy dairy cows were use in this study, genomic DNA was extracted from the blood of dairy cow, PCR-RFLP was applied to genotype of these DNA samples. Analysis of Variance (ANOVA) was performed by the General Linear Model (GLM) procedure of SAS 9.13 to identify the association of SNP with milk and reproductive traits. Results showed that SNP in LOC514211 was polymorphic in this herd of dairy cow and was in Hardy-Weinberg disequilibrium, and had significant differences among genotype in milk yield, milk quality and reproductive traits, which were similar with the results of previous GWAS study based SNP-Chips. Inconclusion, indicated that this SNP in LOC514211 might be potential markers for both milk and reproductive traits.
Author: A. S. Anggraeni, E. Kurnianto, S. Johari, S. Sutopo, Z. Shujun
Journal Code: jppeternakangg150061