Level of Circulating Endothelial Cells and Expression of Nuclear Factor Kappa Beta of Human’s Peripheral Blood Mononuclear Cells in Subjects with Certain Conditions
ABSTRACT: to detect the levels
of CEC and expression of NFκB in the three groups of subjects with certain conditions.
Methods: this study is an exploratory study using human peripheral blood
samples. The study subjects comprised three groups, the group of 23 healthy
people, a group of 35 people that has one or more risk factors for vascular
disease and a group of 15 vascular disease patients (coronary heart disease,
diabetes mellitus, stroke). CEC were isolated from peripheral blood mononuclear
cells (PBMC). CEC level is identifed through the measurement of CD45 and CD146
by flowcytometry method. NFκB expression is recognized by ELISA method (imgenex,
USA). Results: the highest average levels of CEC were found in the sick group
(28.6%). The highest average expression of NFκB (924.9495) is found in the
group with risk factors. The lowest average expression of NFκB and CEC is found
in the healthy group. Statistical analysis of ANOVA at the interval confdence
of 95% shows a signifcant difference (p=0.00) levels of CEC and NFκB expression
between the healthy group with the group with the risk of cardiovascular
disease (CVD) and patients with known CVD. Conclusion: increase of level CEC
and NFκB expression has a strong relationship with vascular disease and its
risk factors.
Key words: circulating
endothelial cells (CEC), nuclear factor kappa beta (NFκB), peripheral blood mononuclear
cell (PBMC), vascular disease
Author: Titin A. Wihastuti,
Djanggan Sargowo, Achdiat Agoes, Satuman, Teuku Heriansyah
Journal Code: jpkedokterangg140269
