Kajian Produksi Aflatoksin B1 Kasar dari Isolat Kapang Aspergillus Flavus Lokal pada Media Jagung dan Jagung+Kacang Tanah
Abstract: Aflatoxin, which
known as toxigenic compound, is a secondary metabolite produced by Aspergillus
flavus and A. parasiticus. Aflatoxin is found in both food and feed stuffs,
such as: corn and peanut. Aflatoxin standard is needed in every research of
aflatoxin contamination. But it is rather difficult to get. It is imported,
high costs, and take times. The aim of the research is to assess the potential
local isolate of A. flavus to producing aflatoxin that can be used to be the
alternative aflatoxin standard. Local mold of A. flavus FNCC 6122 and FNCC 6109
are got from PAU University of Gadjah Mada. Isolate was enriched on PDA medium
for 5 days and move to corn medium of corn+peanut combine medium for 10-15 day
at 25ºC. The variables was the content of AFB1 by ELISA test. The result is
shown that isolat FNCC 6122 produced higher AFB1 than isolate FNCC 6109. The
combine of corn and peanut medium stimulated FNCC 6122 to produce higher AFB1
and has potency to be the candidate of standard aflatoxin. In conclusion, the
peanut addition on the medium would initiate increasing of the aflatoksin B1
level.
Penulis: listya purnamasari,
Ali Agus, Cuk Tri Noviandi
Kode Jurnal: jppeternakandd160078
