In vitro Fermentation Characteristics and Rumen Microbial Population of Diet Supplemented with Saccharomyces cerevisiae and Rumen Microbe Probiotics
Abstract: The objective of
this study was to select three strains of probiotic Saccharomyces cerevisiae
and to evaluate the effect of S. cerevisiae and rumen bacteria isolate (MR4)
supplementation and their combination on rumen fermentability and rumen
microbial population. Experiment 1 was designed in a 4 x 5 factorial randomized
block design with 3 replications. The first factor was S. cerevisiae strain
consisted of control treatment (without S. cerevisiae supplementation), NBRC
10217, NRRL Y 567 and NRRL 12618, and the second factor was incubation time
consisted of 0, 1, 2, 3, and 4 h. Ration was basal ration for feedlot with
forage to concentrate ratio (F:C)= 60:40. Dosage of each treatment with S.
cerevisiae was 5 x 1010 cfu/kg ration. Experiment 2 was designed in randomized
block design with 4 treatments: P0= basal ration of feedlot; P1= P0 + S.
cerevisiae; P2= P0 + MR4 isolate (5 x 107 cfu/kg ration); P3= P0 + S.
cerevisiae and MR4 isolate. The result of experiment 1 showed that supplementation
of S. cerevisiae NRRL 12618 had the highest S. cerevisiae population and
increased rumen bacterial population. This strain was selected as probiotic in
experiment 2. The result from experiment 2 showed that probiotic
supplementation stabilized rumen pH and produced the highest NH3 concentration
(P<0.05) and bacterial population (P<0.05). As compared with control, all
treatments reduced protozoa population (P<0.05). Combination of S.
cerevisiae and MR4 probiotics produced the highest total volatile fatty acids
(VFA) and isovalerate (P<0.05). It was concluded that strain S. cerevisiae
NRRL 12618 had potential as probiotic yeast. Supplementation with this strain
increased fermentability, rumen isoacid and decreased A:P ratio. Those
abilities could be improved with MR4 rumen isolate probiotic.
Author: Lilis Riyanti, Suryahadi,
Dwierra Evvyernie
Journal Code: jppeternakangg160021