Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzymes from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation
Abstract: This research was
aimed to evaluate the effect of inoculant and crude enzyme of Trichoderma
reesei on fermentation quality and rumen digestibility of napier grass silage.
This research consisted of two stages. The first stage was fermentation quality
using a completely randomized design with three treatments and six
replications. The second stage was rumen fermentability using a randomized
block design with three treatments and six replications. The treatments of both
stages were: T0= control of napier grass silage (Hi-fer+), T1= control + T.
reesei inoculant at the level of 2.13 x 107 cfu/kg, and T2= control + crude
enzyme from T. reesei at the level of 11.4 unit/kg. Napier grass silage were
kept at room temperature and opened 21 d after fermentation. The result of the
first stage experiment showed that T2 decreased (P<0.01) dry matter, organic
matter, crude fiber, and neutral detergent fiber (NDF) and also increased
(P<0.05) crude protein, total digestible nutrient (TDN), pH, acetic acid,
and propionic acid of napier grass silage while T1 decreased (P<0.01) NDF
and increased (P<0.05) propionic acid. The result of the second stage
experiment showed that there were no significant differences in rumen pH, in
vitro dry matter and organic matter digestibility, total VFA, proportion of
VFA, ratio of acetic to propionic acids, and also NH3. It is concluded that the
addition of crude enzyme from T. reesei could improve fermentation quality of
napier grass silage with decreasing NDF and increasing TDN but did not affect
rumen fermentability.
Author: Dea Justia Nurjana, S.
Suharti, . Suryahadi
Journal Code: jppeternakangg160026