IDENTIFICATION OF UTERIN MILK PROTEIN (UTMT) GENE IN BALI CATTLE USING DIRECT SEQUENCING
Abstract: The objective of
this research was to identify diversity of exon 5 UTMP gene fragment in Bali
cattle using direct sequencing. The total 60 blood samples of Bali Cattle
derived from BPTU Bali in Bali siland (20 heads), BPTU Serading in Sumbawa
island (20 heads) and Village Breeding Center in Barru District South Sulawesi
(20 heads) were used to evaluate their genetic diversity at exon 5 UTMP gene.
The forward and reverse data sequences were analyzed using Bioedit program and
alignment analysis was carried out using MEGA5 program. Meanwhile haplotype
analysis was performed by DnaSPv5 program. The result showed that partial sequences
in exon 5 UTMP gene had 16 haplotypes with the highest number of haplotypes
ware found in VBC Barru district South Sulawesi (8 haplotypes). Moreover, the
highest average of haplotype (h) and nucleotide (p) diversity were found in VBC
Barru district South Sulawesi were 0.7949 and 0.0016, respectively. In
addition, minisatellite insersion was found in exon 5 UTMP gene fragment on
Bali cattle which are consist of 5'-CCA GTC ATG AAG AAG GCA GAG GTC GTC GTG CCG
GCG AAA-3'. According to our results, haplotype and minisatellite variation in
exon 5 UTMP gene fragment can be used as a candidate genetic marker specific
for reproductive trait in the Bali cattle and for its strategy breeding program
in the future.
Author: J. Jakaria, F.
Saputra, K. A. Paramitasari, P. P. Agung, M. Maskur
Journal Code: jppeternakangg160016
