Honey improves lipid profile of diet-induced hypercholesterolemic rats
Abstract: Coronary heart
disease (CHD) is a major cause of morbidity and mortality throughout the world,
including Indonesia. One of the risk factors for CHD is hypercholesterolemia.
One of the natural products that has been developed for the treatment of
hypercholesterolemia is honey. Honey contains fructooligosaccharides, various
vitamins, minerals, and enzymes which are supposedly able to lower blood
cholesterol levels. This research aimed to study the influence of honey on the
levels of blood total cholesterol, triglyceride, and low density lipoprotein
(LDL) levels in Wistar rats.
This study was of experimental post test control group design.
Twenty-four male Wistar rats (Rattus norvegicus) were randomly divided into 4
groups. K1 was the negative control group (with normal diet), K2 was the
positive control group (with high-fat diet), P1 was fed a high-fat diet for 7
days, followed by high-fat diet plus honey for the next 7 days. P2 was fed a
high-fat diet for 7 days, followed by regular diet plus honey for the next 7
days. After completion of this treatment, total cholesterol, triglycerides, and
LDL levels were measured by the cholesterol oxidase phenol+aminophenazone
(CHOD-PAP) method using enzymatic spectrophotometry principles.
There were significant differences in total cholesterol, triglyceride,
and LDL levels between all groups after day 15 (p<0.05).
Honey supplementation was able to reduce the blood levels of total
cholesterol, triglycerides, and LDL. Honey supplementation accompanied by
non-cholesterol feeds could more effectively lower total cholesterol,
triglycerides, and LDL serum levels in Wistar rats.
Author: Titis Nurmasitoh,
Miranti Dewi Pramaningtyas
Journal Code: jpkedokterangg150284
