Effects of Temperature, Relative Humidity, and DEN-2 Virus Transovarial Infection on Viability of Aedes aegypti

Abstract: Environmental changes influenced survival life and virus transmission of dengue virus (DEN) in a mosquito. The purpose of the present study was to define DEN-2 virus transmission dynamic and effect of temperature, relative humidity (RH), and DEN-2 virus infection on viability of Aedes aegypti (Ae. aegypti). This experimental study with pretest-posttest control group design was conducted at the Laboratory of Center for Tropical Medicine, Faculty of Medicine, Gadjah Mada University (UGM), Yogyakarta. Seventh daysold female Ae.aegypti (F0) were infected DEN-2 viaoral membrane and kept until F2 generation by transovarial transmission, number of eggs produced and hatched was recorded. After 14-day incubation was found that trans ovarial transmission rate of DEN-2 virus infection in F0 and F1 were 93.3% and 82.2%, respectivel y. Egg production, hatchingrates from infected and uninfected mosquitoes F0 were 68% and 85%; and F1 were 72.6% and 76%, respectivel y. At defined room condition tests, 7 day adult mosquitoes in dark and humid environment produced highest number of eggs, compared normal environment and in incubated without CO 2. In fourteenth day oldmosquitoesat dark and humid produced highest number of eggs, compare normal environment condition, and in incubated without CO2. DEN-2 virus was able to infect Ae.aegypti by transovarial transmission where the infection rate in F0 was higher than F1 generation. Temperature and humidity affected the abilityof Ae. aegypti eggs to live and grow to adulthood.
Keyword: Aedes aegypti; DEN-2 virus; humidity; temperature; transovarial; virus DEN-2; kelembaban; suhu
Penulis: Tri Baskoro T. Satoto, Sitti Umniyati, Adi Suardipa, Margareta Sintorini
Kode Jurnal: jpkesmasdd130502