Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D
ABSTRACT: Curcuma zedoaria is
one of the herbal plants potentially protects and cures many diseases,
particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it
are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore
the cytotoxicity of Curcuma zedoria extract (CZE).
Methods: Experimental Quasi with post test non equivalent control group
design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung
Islamic University, Semarang. The research was consisted of 2 groups, namely:
intervention group with CZE zam-zam solvent and intervention group with CZE
ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250
μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL;
1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed
by probit analysis. Results: IC 50 value of CZE in zam-zam and ethanol solvent
were 28.24 μg/ml and 13.71 μg/ml respectively at the same 24 hours incubation
period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that
there was significant relationship.
Conclusion: CZE activity using ethanol is highly active while CZE using
zam-zam water is moderate and both of them have the toxicity on breast cancer cell.
As the result, apoptosis process may occur.
Author: Isna Hudaya,Taufiq R
Nasihun, Titiek Sumarawati
Journal Code: jpkedokterangg150294
