EFEK PEMBERIAN CHLOROPHYLLIN TERHADAP KADAR NITRIC OXIDE DAN MALONDIALDEHIDA TIKUS HIPERKOLESTEROLEMIA
ABSTRACT: Endothelial
dysfunction is well-known as an early stage of atherosclerosis. Increased
oxidative stress inhypercholesterolemia triggers endothelial dysfunction that
is characterized by decreased biological availability ofnitric oxide (NO).
Experimental studies showed that chlorophyllin had an effective antioxidant
activity.Objectives : To determine the effect of chlorophyllin on nitric oxide
(NO) and malondialdehyde (MDA) level ofhypercholesterolemic rats.Methods : Post
test only randomized control groupusedtwenty four male Sprague Dawley rats were
randomly dividedinto four groups: without treatment (technical control/K1),
hypercholesterolemic (negative control/K2),hypercholesterolemic with
chlorophyllin at dose of 1,8 mg/200 g/d (P1) and hypercholesterolemic with
chlorophyllin atdose of 3,34 mg/200g/d (P2). Chlorophyllin was dissolved in
water and was given via nasogastric tube for 21 days afterthe rats got
hypercholesterolemia. Total cholesterol level of blood was measured by CHOD-PAP
method, level of NOplasma was measured by Griess reaction and level of MDA
plasma was measured by TBARS method. Hypothesis testwas analyzed by One Way
Anova continued by Post hoc LSD test and Kruskall Wallis by significant level
of 0,05.Results :NO plasma level was the lowest in group P2 (0.203+0.015 μM)
and the highest in the group K2 (0,224±0,001μM), but no difference of NO plasma
level among groups (p = 0.118). There were differences in MDA plasma levelamong
the groups (p = 0.001). P1 and P2 groups had lower MDA plasma level than K2
(2.40+0.11 nmol/ml), indicatedby (1.94 +0.07 nmol/ml, p=0.0001) and (1,37 +0.13
nmol/ml, p=0.0001) respectively, but still higher than K1(0.94+0.05 nmol/ml,
p=0.0001).Conclusion : The treatment of chlrophyllin does not have an effect of
NO plasma level, but gives an effect of lowerMDA plasma level.
Penulis: Galeh S Pontang, Andrew
Johan, Hertanto W Subagio
Kode Jurnal: jpkesmasdd140409
