Diagnostic Study on Identification Method of Enterobacteriaceae Directly from Blood Culture
Abstract: The provision of
rapid diagnosis results from positive blood cultures is important for clinical
management of sepsis. Using conventional method as a reference method in
laboratory, time needed for bacterial identification are 24 hours longer
because it has to deal with primary isolation step. Objectives: This study
investigated the accuracy of direct inoculation technique of bacteria from
positive blood culture vials to biochemical test tubes without primary
isolation step to identify Enterobacteriaceae, second most common causative
agent of sepsis. Methods: The study was conducted at the Microbiology
Laboratory Medical Faculty of Diponegoro University. This is a diagnostic
study. As the study sample, blood cultures in BACTEC bottles from Dr. Kariadi
General Hospital Semarang with bacterial growth in it. Inclusion criteria was
Gram-negative rod bacteri, staining results from BACTEC blood culture bottles,
and as a exclusion criteria, there are more than one colony found on blood agar
and Mac Conkey agar and showed positive result in oxidation test.
Identification of bacteria based on biochemical table of Enterobacteriaceae.
Data were analyzed with a 2x2 table. Results: Thirty two samples included in
this study. Ten samples (31%) were excluded. Twenty one from twenty two (95%)
study samples accurately identified to the genus level by direct inoculation
method. Conclusion: The results showed that the direct inoculation method
provides an acceptable genus identification, with a potential saving of 24
hours compared with conventional methods.
Author: V. Rizke Ciptaningtyas,
Rebriarina Hapsari, Tri Nur Kristina, Winarto
Journal Code: jpkedokterangg150292
