DIAGNOSIS MOLEKULER HUMAN PAPILLOMA VIRUS (HPV) PENYEBAB KANKER SERVIKS
ABSTRACT: Cervical cancer is
still the one of major public health problem in the world whether an effective
screening programs using the Papanicolaou test to detect precancerous lesions
has reduced the number of cases of cervical cancer in the developed countries
but not in developing countries. The sensitivity and specificity of the Pap
smear depends on observer’s skills to recognize and classify variations of
abnormal cells.
Human Papilloma Virus (HPV) caused cervical cancer has been proved, In some study have found HPV DNA in 99.7 % of
all cervical carcinomas. HPV has been found to be more than 100 genotypes ,
which are divided into high-risk HPV and low-risk HPV
The development of early diagnosis for the detection of HPV infection
still has been a problem, where the cytology and colposcopy to identify lesions
at an advanced stage, therefore the molecular engineering approach seems to be
more successful in early diagnosing cervical cancer. This technique of
molecular HPV DNA sequences are Southern Blot Hybridization, Hybrid Capture II
Assay, Polymerase Chain Reaction, and PCR - Reverse Line Blot. The Accuracy of
molecular diagnostic techniques is required to provide further information for
patient how the management and maintenance of cervical cancer.
Penulis: Lipinwati
Kode Jurnal: jpkedokterandd140587
