Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation
Abstract: Cell proliferation
occurs not only in normal but also in cancer cells. Most of cell proliferation
inhibition can be done by inhibiting the DNA synthesis, notably by intervening
the formation of purine or pyrimidine. In purine de novo synthesis, it was
assumed that biotin plays a role as a coenzyme in carboxylation reaction, one
of the pivotal steps in the purine de novo pathways. The aim of this study was
to see the avidin potency to bind biotin and inhibit mitosis.
Methods: Peripheral blood mononuclear cell (PBMC) was cultured in
RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or
absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin
addition was observed at 24, 48, and 72 hours for cell proliferation,
viability, and cell cycle. Statistical analysis was done by one-way ANOVA.
Results: Avidin inhibited cell proliferation and viability in culture
under stimulation by PHA with and without IL-2. Cell cycle analysis showed that
avidin arrested the progression of PBMC after 72 hours of culture. Most cells
were found in G0/G1 phase.
Conclusion: Inhibition of biotin utilization by avidin binding can halt
cell proliferation.
Author: Cicia Firakania, Indra
G. Mansur, Sri W.A. Jusman, Mohamad Sadikin
Journal Code: jpkedokterangg160135