Association ofTumor Necrosis Factor Alpha andLymphotoxin Alpha Gene Polymorphisms with the Presence of Chronic Obstructive Pulmonary Disease
ABSTRACT: to analyze the
occurrence of chronic obstructive pulmonary disease (COPD) and its association
with the polymorphisms of -308G/A, -238G/A of TNFα gene and +252A/G LTα gene
polymorphism in smokers. Methods: cross-sectional study, comparing the genetic
group of people who have COPD and who do not have COPD with the same smoking
history. The study was conducted from January 2011 to March 2012 at several health
centers; such as Adam Malik Hospital, Pirngadi Hospital, Tembakau Deli
Hospital, Siti Hajar Hospital in Medan and several health centers in the city
of Medan. Examination of lung function was done using spirometry and the
existence of genetic polymorhisms in the TNFα and LTα gene was performed using
polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP).
Results: of the total of 227 samples that met inclusion criteria and after
equalizing the age and history of smoking, 186 subjects were enrolled, of
which, 93 people as COPD group and 93 people as non-COPD group. Analysis of the
association between the -308G/A polymorphism with the COPD revealed the odds
ratio (OR) 0.436 (95% CI 0.224-0.850, p=0.014). Polymorphism at -238G/A of the
TNFα gene showed the OR 2.094 (95% CI 0.608 - 7.211, p=0.241). Polymorphism at
+252A/G LTα gene showed OR 1.256 (95% CI 0.694 - 2.272; p=0.450). The fndings
indicated that polymorphism at -308G showed a protective factor whereas the
-238G/A of the TNFα and +252A/G of the LTα genes did not show any signifcant
association with the COPD. Conclusion: polymorphism -308 TNFα gene shown to be
a protective factor for the occurrence of COPD. Polymorphism -238 TNFα gene and
+252 LTα gene did not show any signifcant association with COPD.
Author: Amira P. Tarigan,
Tamsil Syafuddin, Faisal Yunus, Suradi
Journal Code: jpkedokterangg150239
