Association of Calpastatin (CAST) Gene with Growth Traits and Carcass Characteristics in Bali Cattle
Abstract: Calpastatin (CAST)
gene is well known as an inhibitor of muscle protein degradation and relates to
muscle growth and meat tenderness. The objective of this study was to determine
the association of CAST gene with growth traits and carcass characteristics in
Bali cattle. A number of data from 35 Bali bulls were collected from BPTU Bali
Cattle to obtain growth traits, carcass characteristics, and blood samples.
Polymorphism of CAST gene in Bali bulls was analyzed by using PCR-RFLP and DNA
sequencing. The association of CAST gene with growth traits and carcass
characteristics were analyzed by using General Linear Model (GLM). The result
showed that there were two genotypes (GG and AG) of CAST gene with allele
frequencies of 0.857 and 0.143, respectively, for G and A. Notably, mutation A
to G occurred in 253 bp CAST fragment gene in Bali Cattle. Genotypes GG and AG
of CAST gene significantly affected (P<0.05) the back-fat thickness and
longissimus dorsi without a significant effect on the growth traits. It could
be concluded that CAST gene had a potency as a marker gene for carcass quality
in Bali cattle.
Author: R. Putri, R. Priyanto,
A. Gunawan, Jakaria
Kode Jurnal: jppeternakangg150042
