Altered expressions of endothelial junction protein of placental capillaries in premature infants with intraventricular hemorrhage
Abstract: Placental hypoxia
may lead to oxidative stress, which inflicts damage to capillary protein
junction. The aim of this study was to evaluate altered expression of
endothelial junction protein of capillaries in hypoxia condition and to observe
its correlation with the incidence of
intraventricular hemorrhage in premature infants.
Methods: A cross-sectional study was conducted by using placental tissues
of premature infants as amodel of capillary integrity (29 hypoxic and 29
non-hypoxic). Hypoxia inducible factor (HIF)-1α was measured to define
placental tissue response to hypoxia; malondialdehyde (MDA) and glutathione
(GSH) served as markers of oxidative stress. The expressions of junctional
proteins, N-cadherin and occludin were analyzed by immunohistochemistry.
Intraventricular hemorrhage (IVH) was detected by cranial ultrasound at the
third day. Unpaired t test, Mann-Whitney, and Chi-square tests were used to
analyze the data.
Results: The HIF-1α and MDA levels were slightly, but not significantly,
higher in hypoxia group {13.64±8.70 pg/mg protein and 10.31 pmol/mg tissue
(ranged 1.92–93.61), respectively}
compared to non- hypoxia group {10.65±5.35 pg/mg protein and 9.77
pmol/mg tissue (ranged 2.42–93.31)}. GSH levels were not different in both
groups (38.14 (ranged 9.44–118.91) and
38.47(ranged 16.49–126.76) ng/mg protein, respectively. mRNA expression
of N-cadherin (0.13) and occludin (0.096) were significantly lower in hypoxia
comparedto non-hypoxia group (p=0,001), while protein expression of N-cadherin (3.4; 75.9; 6.9; 13.8%) and
occludin (20.7; 3.4; 69.0; 3.4;
6.9%) in hypoxia group was not
associated with IVH (p=0.783 and p=0.743).
Conclusion: Hypoxia altered expression of endothelial junction protein in
placental capillaries, but no association with intraventricular hemorrhage was
observed.
Author: Maria Ekawati, Ninik
Mujihartini, Ahmad A. Jusuf, Nani Dharmasetiawani, Sri W.A. Jusman, Mohamad
Sadikin
Journal Code: jpkedokterangg160141
