AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA
Abstract: Belimbing wuluh
(Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties.
The purpose of this study was to test the antibacterial activity of bark
Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis
and their bioautography. Extraction methods used to research is method
maceration with a solvent ethanol 96 %. Fractinations done by method partition
liquid-liquid with a separating funnel. Test performed in this research
covering identification
bacteria, the sensitivity bacteria, antibacterial activity, thin layer
chromatography, bioautography. The result of antibacterial activity ethanol
extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk,
1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae,
10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with
concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29;
10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus
epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800
μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella
pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis,
ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600
μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29;
10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds
contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction,
and ethanol-water fraction is a compound of the saponins, alkaloids, avonoids and phenolic. Bioautography showed
that ethanol extracts, n-heksan faction, ethyl acetate fraction, and
etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not
antibacterial activity because there is no clear area around on plate TLC.
Keywords: Belimbing wuluh
(Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial,
bioautogra
Penulis: Dr. Muhtadi , MSi.,
Ria Ambarwati, Ratna Yuliani
Kode Jurnal: jpkedokterandd120211