UTILIZATION OF cpDNA SEQUENCES TO IDENTIFY 15 MANGO ACCESSIONS
Abstract: Information about
genetic relationship and genetic diversity are an inevitable need in mango
breeding program. Base substitution value, genetic distance and grouping of 15
mango accessions based on accessions in chloroplast DNA (cpDNA) among 15 mango
accessions were assessed. The samples were originated from Mango Germplasm,
Cukur Gondang Research Station of Indonesian Tropical Fruits Research
Institute, Pasuruan, East Java. Sequencing of Chloroplast DNA was used to
obtain nucleotide sequence data. Paired specific primer rpl20 F - rps12 R and
atp F - rbcL R were used for amplification of non-coding area of mango DNA
chloroplast and sequencing processes as well. All data were analyzed using
Software MEGA 6. The result showed that total numbers and nucleotide base
sequences varied among all accessions. All accessions were grouped in five
different clusters that might be used as source of parental breeding.
Author: Sukartini, Taryono
Taryono, Panjisakti Basunanda, Rudi Hari Murti
Journal Code: jppertaniangg160012
