Uji Daya Hidup Bakteri Asam Laktat Dari Usus Itik Pada Media Tumbuh Dengan Penambahan Variasi Konsentrasi Molasis
Abstract: This study aims to
determine differences in the viability of Lactic Acid Bacteria (LAB) isolates
on MRS medium supplemented with various concentrations molasses. Concentration
variation molasis used as controls were 0%, 1%, 2% and 3%. Variation of time
incubation on each of the media that is 0 hours, 24 hours, 48 hours and 72 hours.
The viability of each isolate was viewed with the population of Log (cells/ml)
which grow on the medium and the number of generations and time of generations.
The results showed that the concentration molasses added to MRS affect the
viability of LAB. Concentration of 1% makes LAB better viability than controls.
Total population continues to increase at the end of the incubation with a
yield of 1.17 generations in time 20.46 hours/generation. In contrast to
controls, reduced population size at the end of the incubation so as not to
produce a generation. LAB viability in addition molasses 2% and 3% live on 48
hours of incubation. This is indicated by the number of population increased at
48 hours of incubation, and then decreased at the end of incubation. The
addition of concentration variation in the types of bacteria showed different
viability. At B1 adding molasses 2% and 3% live on 48 hours of incubation and
control can only live on a 24 hours incubation, whereas the B3, the control can
remain alive at 48 hours of incubation. In contrast to the controls on B4 can
survive on 48 hours of incubation, whereas the addition of 3% molasses can only
live on a 24 hours incubation.
Penulis: Rudy Sutrisna,
Christina Nugroho Ekowati, Riska Damayanti
Kode Jurnal: jppertaniandd160242