POTENSI ANTELMINTIKA EKSTRAK BAKTERI SIMBION SPONS LAUT TERHADAP TRICHOSTRONGYLIDAE (NEMATODA) PARASIT DOMBA
ABSTRACT: Trichostrongylidae
(nematodes) parasitic infection of sheep were recently resistance to
anthelmintics. Bioactive utilization of bacteria derived sponges had potential
as anthelmintics alternative naturally against trichostrongylidae parasitic
infections. The Aims of this study was to determine the activity of bacteria
derived sponge extracts which produced anthelmintics bioactive compounds
against sheep trichostrongylidae parasite. Bacteria derived sponges isolates
which labeled S1 and S2 were extracted by methanol. Phytochemical test were
conducted to determined characterization of bioactive compounds which
potentially to inhibit larvae migration. Concentration which used to Larva
Migration Inhibition Assay (LMIA) were 25, 50, 100, 250, and 500 µg/ml.
Positive control treatment was used albendazole while negative control by
physiological of NaCl. Both of extracts were contained toxicity to againts
Artemia salina larvae which <1000 µg/ml concentration. The higher
concentration of S1 and S2 extracts were affected to increase larvae migration.
Both of extracts were potential to inhibit larvae migration which LC50 value
were 165.63 µg/ml (S1) and 374.9 µg/ml (S2). The ability of extracts which
inhibit larvae migration caused by bioactive compounds which contained
triterpenoids in both of extracts then flavonoid compounds only by S1. Albendazole
was showed a highest inhibitory activity which contained 95.5% of the total
test nematode larvae.
Penulis: Muhammad Reza Faisal,
Mujizat Kawaroe, Fadjar Satrija
Kode Jurnal: jppertaniandd160108