PENGGUNAAN MARKA DNA DALAM SELEKSI INDUK IKAN KERAPU MACAN (Epinephelus fuscoguttatus) YANG TAHAN TERHADAP BAKTERI Vibrio alginolyticus
ABSTRACT: The objective of the
present study was to use selected RAPD markers (Primer UBC-122 and YNZ-22) in
parental selection to the bacterial Vibrio alginolyticus resistance in Tiger
groupers (Epinephelus fuscoguttatus). Eighteen fish parent samples were
challenged with Vibrio alginolyticus and parental selection was examined by a
PCR-RAPD method. The results showed that from 18 fish samples, primer YNZ-22 generated
18 fragments while primer UBC-122 generated 8 fragments only (KHP, KB, HB, PB,
MKH, MH, K and MK). In addition, the average number of fragments from each
samples generated from primer-YNZ was higher (3,2) than from UBC-122 (2,3).
This indicates that primer YNZ-22 is better than primer UBC-122 to use in
parental selection to the Vibrio alginolyticus resistance in Tiger groupers.
From the parental selection, five fish parents (KHP, PB, MH, K and MK) were
selected for their high resistance to the bacterial Vibrio alginolyticus.
Penulis: St. Hidayah Triana,
Mahir S. Gani, Asmi Citra Malina A.R. Tassakka dan Hamka
Kode Jurnal: jpperikanandd140194
