Penggunaan Ekstender Madu yang Dikombinasikan dengan Krioprotektan Berbeda pada Pengawetan Sperma Ikan Nilem (Indonesian Sharkminnow, Osteochilus hasseltii Valenciennes, 1842)
ABSTRACT: Sperm
cryopreservation is beneficial for aquaculture industry (e.g. maintaining
genetic variability of broodstock, efficient utilizing of sperm and
synchronizing of artificial reproduction) and for ex-situ conservation (gene
banks of endangered species, indigenous organism and valuable strain). Nilem is
an representative model for investigating basic cryobiological parameters and
developing a cryopreservation procedure for commercially cultured fish and
endangered species in the cyprinid. The experiment was conducted to investigate
effective combination of honey as an extender with dimethyl sulfoxide (DMSO) or
methanol as cryoprotectants. Milt was diluted in extender (honey 0,5%) at the
ratio of 1:9 then cryoprotectant was added at 5%, 10% or 15% (v/v)
concentrations. Samples were stored in 0,5 mL straws, equilibrated at
temperature 4-5 oC for 20 minutes, vaporized at 3 cm above surface liquid nitrogen
for 7 minutes and then plunged into liquid nitrogen, where they were stored for
2 weeks. Sperm was thawed at temperature 39-40 oC for 10-15 seconds and was
used to fertilize 100-200 eggs per straw. The percentage of sperm motility of
pre-freezed sperm was not significantly different in all treatment and the highest
post-thawed sperm motility was the combination of honey extender and DMSO 15%
(63,33%). Sperm cryopreservation using honey with DMSO 15% resulted in highest
hatching rate (87,97%). The utilization of honey in combination with DMSO
proved to be suitable for cryopreservation of nilem sperm, especially with
regard to sperm motility and success of hatching from fertilized egg by
frozen-thawed sperm.
Penulis: Ade Sunarma, Dewi
Wisudiyanti Budihastuti dan Yulia Sistina
Kode Jurnal: jpperikanandd100162